Topic · 24 posts
Nikon
24 posts on Nikon, 2009–2025, newest first.
Nikon posts, newest first
- 15 Oct2025
Nikon Ti-E EPI Filter Wheel
What to look for and how to replace the drive gear The Venerable Nikon Ti-E remains in service in research labs all over the world. One of the first autofocus-enabled instruments deployed with the integrated PFS, there isn’t much to ask…
Device automationMicroscopesNikon1 min read
- 15 Jul2016
Microscope Objective Zemax Files
While I haven’t had a chance to test these for accuracy, I was excited to find them while looking for some tube lens specs! You can find the file set here. These designs include some of the common infinity corrected objectives, as well as…
ImagingMicroscopesNikon1 min read - 16 Jul2012
STORM comparison from UCSF NIC
I recently received a beautiful comparison image produced by the now operational STORM system at the UCSF NIC.The sample was prepared by Michael Davidson, and was imaged by Kurt Thorn of the NIC. Click the image for a higher res view…
NIS-ElementsNikonSuper Resolution1 min read
- 02 Mar2012
Nikon nSIM wins #5 in TheScientist’s 2011 Best and Brightest
Here’s a short video review of the top 10 innovations in 2011. Some really cool technology here! Top 10 Innovations of 2011 from thescientistllc on Vimeo.
Device automationImagingNikon1 min read - 30 Jan2012
How TIRF works on a Microscope
TIRF is a widely used tool for effectively creating a super resolution instrument out of a standard widefield microscope. The cool part of TIRF is that it bypasses the limits of axial resolution on the scope not by altering the optics…
ImagingNikonTIRF3 min read
- 07 Nov2011
WHY YOU SHOULD ATTEND THE UCSF/QB3 Microscopy Course
Over the course of my career, I’ve flown across the US to attend a variety of imaging related instructional courses, all on the eastern seaboard. We are all familiar with the Woods Hole courses, the AQLM course, and so forth. What has…
NIS-ElementsImageJImaging1 min read - 11 Oct2011
Coupling a C-2 confocal to an AZ-100 zoom scope
I recently worked on a demo where the customer wanted a confocal with magnification range from macro (1x) to low power compound levels (200-400x). In order to meet this requirement we showed him the AZ-100 zoom scope, and the C-2 scanning…
Nikon1 min read
- 12 Sep2011
Updating Software? A pre-chaos checklist…
A few years after your shiny new system is installed, you’ll inevitably look at it just like you look at a car. What was once running flawlessly now has a few bugs, some dust, is slower and generally worn in. So, at some point you’ll…
NIS-ElementsImageJMicro-Manager5 min read - 29 Aug2011
PhotoFluor 2 Driver for Nikon Elements
89 North has asked me to cook up a driver for the PhotoFluor 2, which I am now hosting under my Macros & Journals page, or you can use this link. This driver works in NIS Elements AR, and BR with Advanced Interpreter. Please contact me if…
NIS-ElementsImagingMacros1 min read - 17 Jun2011
Nikon Oils, Perfect Focus and What to Use
There are two docs published by Nikon regarding the use of oils. Most people think you can use any old oil for any microscope, but this is not the case. Zeiss, Leica, Nikon and Olympus all have specific chromatic, spherical and viscosity…
ImagingNikon1 min read
- 03 Jun2011
Ti-E Perfect Focus Usage and Troubleshooting
The Ti-E PFS is an excellent automatic focusing system. Using the system hinges on one key aspect: The NIR LED used for focusing. The PFS is basically a laser reflection system. A near IR LED is delivered into the back of the objective…
Nikon3 min read
- 29 Apr2011
Voltage output from NIS Elements, MetaMorph or uManager for under $200
I posted briefly about this a while back, in my explanation of the demise of the parallel port. I finally got my hands on a National Instruments USB-6008 box, and have found the unit to be 100% compatible with any NI-DAQ controlling…
Device automationNIS-ElementsImageJ2 min read
- 18 Apr2011
Nikon C2 – NIS Elements and Spectral Capability
Nikon has redesigned and updates it’s classic C1 Confocal. The C1 was a great core facility confocal as it was a stable and reliable product. The one thing that held it back was the lack of access to NIS Elements software. With the release…
NIS-ElementsImagingNikon1 min read
- 08 Apr2011
Nikon DS U3 Series Controller Install Guide
The new Nikon controllers for DS Series Cameras run on Firewire. As with other firewire adapted imaging cameras, there are numerous small issues to awtch out for during the setup and operation process. Once they are up and running, they…
Device automationDS CamerasNIS-Elements1 min read - 29 Mar2011
Determining background sources in fluorescence
For microscopy engineers and suppliers like me, the background produced in an image is of importance, if that background is created by the imaging system itself. What so many of us on this side of the scope (vs the user side) forget to…
NIS-ElementsImageJImaging2 min read
- 11 Feb2011
*UPDATED* Micro-Manager Tethered to a Nikon Digital SLR
***UPDATE 3/3/2014*** After a few requests for a report on x64 capability using this tethering technique, I tried installing the NK Remote software on a rather fresh copy of Windows 7×64 – it did not communicate with the camera at all. On…
Device automationDSLR CamerasNIS-Elements3 min read
- 07 Feb2011
Fix for Elements v 3.2 and ASI Stages
As anyone who is running NIS Elements v 3.20 + is aware, the ASI stage controllers of any MS-2000 series will not be usable once elements issues a connection command to the controller. Elements will control the stage properly, however the…
ASI StageNIS-ElementsImaging2 min read - 27 Aug2010
Instensity Calibration using a Photostable Light Source
I’m very excited to announce Technical Instruments’ first ever camera-specific calibration product! At left is an image of the CC-560, or Camera calibrator, which emits photons at 560nm. All of the cameras in the microscopy world rely upon…
Device automationImagingNikon3 min read
- 16 Jul2010
SLR cameras for Microscopy
Most of the time, I work with people who need measurable and accurate (quantitative) results from the images they obtain. While this constitutes the bulk of my daily job, there is a much larger group of people using cameras on microscopes…
Device automationDSLR CamerasImaging4 min read - 17 Apr2010
How to Set Large Icons in Elements
This is useful for large screens that are using very high resolutions. Sometimes the icons are so small as to be hard to click and unreadable. Open Elements and select the View Menu, then Layout, then open the “Layout Manager”. Next select…
NIS-ElementsImagingNikon1 min read
- 05 Mar2010
NIS Elements Stereo Zoom Settings
The Auto-calibration feature in Elements (beginning in version 3.1) is a great thing to have. You simply choose the objective you are working with, the software knows what camera you are running, so the calibration is calculated for you…
NIS-ElementsImagingNikon1 min read - 04 Jan2010
Dynamic Graph Output for Elements
Happy New Year! Laura Sysko from Nikon sent us a How-To on outputting live graphs that can be included with a timelapse or other ND file. You can find the How To Here.
NIS-ElementsImagingNikon1 min read - 21 Sep2009
Elements 3.1 Released
Nikon has released it’s major revision of Elements in version 3.1. (Build 587). There are several new features in this build and from what i’ve seen it’s quite stable. You can download the build here. – Austin
Device automationNIS-ElementsNikon1 min read - 08 Sep2009
DXM-1200, 1200F and 1200 C Camera Compatibility – FAQ
A few years back Nikon released a very cool 12 megapixel stepping camera, called the DXM-1200. The camera was reliable and a nice product for many years. Unfortunately the march of time made a dent in this camera. The system, like many…
DXM cameraDXM chipsetDXM-1200C1 min read