Formulas for microscopy

I had a customer recently ask for these, and I thought I’d post them. These are from Nikon Inc. I’ll also have them on the resources page for reference. These include Useful magnification, Brightness Factor, Refractive Index, Depth of Field at Image Plane, Depth of Focus at Specimen Plane, Useful Magnification and Infinity Optics Formulas.

 Formula Key